Table 1.

Expression of CYP27B1 in hMSCs, Kidney, and Other Tissues/Cells

Tissue	Cell type	RNA	Protein	Activity	References
Bone Marrow	hMSCs	yes	WB	1	[8,9,12]
Kidney	Human kidney	yes	yes	2	[76]
Bone	Bone biopsy	yes	N.D.	N.D.	[39]
	Human primary ostoblast	yes	N.D.	1	[48]
	Osteoclast RAW 264.7 cells	yes	IF	1	[39,50,51]
Human osteoblast-like cells	MG-63	yes	N.D.	1	[39]
	SAOS-2	yes	N.D.	1	[47]
	G-292	yes	N.D.	1	[47]
	HOS	yes	IF	1	[49]
	SV-HFO	yes	WB	1	[39]
Human keratinocytes	Primary human keratinocytes HPK1A cell line	yes	ICC	3	[41,77]
Prostate	Primary normal prostatic epithelial cells	yes	WB	2	[46]
	Cancer-derived prostatic epithelial cells	yes	WB	2	[46]
	Benign prostatic hyperplasia	yes	N.D.	2	[46]
Human prostate cancer cell lines	LNcaP	yes	WB	2	[46]
	PC-3	yes	WB	2	[46]
Colon	Human normal and malignant Colon mucosa and colon tumors	yes	WB IF IHC	N.D.	[42]
Immune system Blood cells	Peripheral blood Mononuclear cells Dendritic cells Macrophages	yes	ICC	2	[42–45]
Human colon cell line	Caco-2	yes	N.D.	3	[78]
	BBe	yes	N.D.	3	[78]
Adipocyte	3T3-L1	yes	N.D.	2	[52]
Others	human umbilical vein endothelial cells	yes	WB IHC	2	[53]
	Brain (cerebellum/purkinje cells)	yes	IHC	N.D.	[42]
	Epidermis (statum basalis)	yes	IHC	N.D.	[42]
	Parathyroid glands	yes	IHC	N.D.	[79]
	Pancreatic islets	yes	IHC WB ICC	N.D.	[42]
	Mammary, breast (cancer or normal)	yes	IHC	N.D.	[80]
	Ovary	yes	IHC	2	[81]
	Human trophoblast cells	yes	N.D.	N.D.	[82]
	Human Vascular Smooth Muscle Cells	yes	N.D.	4	[83]
	Lymph node Spleen cells	yes	IHC	2	[42,84]
	Human small cell lung cancer cell line NCI H82	N.D.	N.D.	3	[85]

RNA: yes means by in situ hybridization, RT-PCR, or real-time RT-PCR;

Protein: detected by Western immonblot (WB), Immunohistochemistry (IHC) or Immuno Fluorescence (IF);

Activity: 1. by radioimmunoassay or enzyme-linked immunosorbent assay kits from IDS; 2. by thin-layer chromatography; 3. by high-performance liquid chromatography; 4. by radioimmunoassay kit from DiaSorin.

N.D.: Not detected.