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. Author manuscript; available in PMC: 2014 Feb 27.
Published in final edited form as: J Am Chem Soc. 2013 Feb 11;135(8):3136–3143. doi: 10.1021/ja310742m

Figure 4.

Figure 4

Screening for the effect of Mg2+ and K+ concentration on Bs SAM-I riboswitch mobility and the extent of the mobility shift in the presence of SAM ligand. All on-chip mobility shifts are statistically significant using a two tailed t-test (p<0.05) and indicated by ‘*’. Relative mobility change is the % increase in RNA mobility in the presence of SAM. WT− and WT+ indicate the absence and presence of SAM ligand, respectively. Error bars are standard deviation of triplicate runs. All runs were done in 1× TB buffer with salt concentrations as indicated in mM.