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. Author manuscript; available in PMC: 2014 Feb 27.
Published in final edited form as: J Am Chem Soc. 2013 Feb 11;135(8):3136–3143. doi: 10.1021/ja310742m

Figure 6.

Figure 6

Microfluidic precision enables mobility shift as metric for screening candidate riboswitch function. The improved resolution of the microfluidic mobility shift assay over conventional slab gel mobility shift assays allows detection of GC-rich putative SAM-I riboswitches. (A) ‘S’ indicates a shift and ‘NS’ indicates no shift. (B) All on-chip mobility shifts are statistically significant using a two tailed t-test (p<0.05) and indicated by ‘*’. Error bars represent standard deviation of triplicate runs. M1, M1N, P2 and P2N mutants do not demonstrate a shift, as expected. See Figure S1 for slab gel data on M1 and P2 mutants. Upper bands in slab gel (‘Δ’) appear to be non-binding RNA conformers. Slab gel E = 8 V/cm, on-chip E = 240 V/cm. 1× TB + 10 mM Mg2+ in gel and run buffers. Relative mobility values are ×10−3 cm2/Vs.