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. Author manuscript; available in PMC: 2014 Apr 17.

Published in final edited form as: Bioconjug Chem. 2013 Mar 26;24(4):571–577. doi: 10.1021/bc300462j

(A) Enzyme activity assay for immobilized His₆-GSTase-CVIA-PE on azide-derivatized glass surface. (B) Plot of GSTase activity as a function of the concentration of enzyme immobilized. His₆GSTase-CVIA-PE - ; and His₆GSTase-CVIA-F - . Estimated GSTase concentration on glass surface (pmole/mm²): A: 5.0; B: 10.0 ; C: 20; D: 40. Activity determined by measuring A₃₄₀ nm in 100 mM NaH₂PO₄ buffer, pH = 6.5, containing 250 μM CDNB and 1 mM GT after incubation for 30 min at 21 °C.

Inline graphic — (A) Enzyme activity assay for immobilized His₆-GSTase-CVIA-PE on azide-derivatized glass surface. (B) Plot of GSTase activity as a function of the concentration of enzyme immobilized. His₆GSTase-CVIA-PE - ; and His₆GSTase-CVIA-F - . Estimated GSTase concentration on glass surface (pmole/mm²): A: 5.0; B: 10.0 ; C: 20; D: 40. Activity determined by measuring A₃₄₀ nm in 100 mM NaH₂PO₄ buffer, pH = 6.5, containing 250 μM CDNB and 1 mM GT after incubation for 30 min at 21 °C.