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. Author manuscript; available in PMC: 2014 Apr 17.

Published in final edited form as: Bioconjug Chem. 2013 Mar 26;24(4):571–577. doi: 10.1021/bc300462j

GSTase activity for covalently immobilized His₆GSTase-CVIA-PE and His₆GSTase-CVIA-F from cell-free homogenates of E. coli M15-pQE(His₆-GSTase-CVIA). Activity was determined by measuring A₃₄₀ in 100 mM NaH₂PO₄ buffer, pH = 6.5, containing 250 mM CDNB and 1 mM GT after incubation for 60 min. at 21 °C. His₆GSTase-CVIA-PE with Cu(I) - ; His₆GSTase-CVIA-F with Cu(I) - ; His₆GSTase-CVIA-PE - ; His₆GSTase-CVIA-F - .

Inline graphic — GSTase activity for covalently immobilized His₆GSTase-CVIA-PE and His₆GSTase-CVIA-F from cell-free homogenates of E. coli M15-pQE(His₆-GSTase-CVIA). Activity was determined by measuring A₃₄₀ in 100 mM NaH₂PO₄ buffer, pH = 6.5, containing 250 mM CDNB and 1 mM GT after incubation for 60 min. at 21 °C. His₆GSTase-CVIA-PE with Cu(I) - ; His₆GSTase-CVIA-F with Cu(I) - ; His₆GSTase-CVIA-PE - ; His₆GSTase-CVIA-F - .