Figure - PMC

Skip to main content

View full-text article in PMC

. Author manuscript; available in PMC: 2014 Apr 17.

Published in final edited form as: Bioconjug Chem. 2013 Mar 26;24(4):571–577. doi: 10.1021/bc300462j

Schematic representation of azide-derivatized GSTase immobilized on alkyne slides. GSTase activity for immobilized His₆GSTase-CVIA-Az and His₆GSTase-CVIA-F from cell-free homogenate of E. coli M15-pQE(His₆-GSTase-CVIA) strain is plotted as a function of product formation. Activity determined by measuring A₃₄₀ in 100 mM NaH₂PO₄ buffer, pH = 6.5, containing 250 μM CDNB and 1 mM GT after incubation for 60 minutes at 21°C. His₆GSTase-CVIA-Az - and His₆GSTase-CVIA-F -

Inline graphic — Schematic representation of azide-derivatized GSTase immobilized on alkyne slides. GSTase activity for immobilized His₆GSTase-CVIA-Az and His₆GSTase-CVIA-F from cell-free homogenate of E. coli M15-pQE(His₆-GSTase-CVIA) strain is plotted as a function of product formation. Activity determined by measuring A₃₄₀ in 100 mM NaH₂PO₄ buffer, pH = 6.5, containing 250 μM CDNB and 1 mM GT after incubation for 60 minutes at 21°C. His₆GSTase-CVIA-Az - and His₆GSTase-CVIA-F -