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. Author manuscript; available in PMC: 2014 Jan 1.
Published in final edited form as: Prog Nucl Magn Reson Spectrosc. 2012 Jun 15;68:41–57. doi: 10.1016/j.pnmrs.2012.06.001

Fig. 9.

Fig. 9

(a) NOESY pulse sequence and the spectrum with RD-driven water flip-back at the start of the mixing time τm. (b) Improved NOESY pulse sequence and spectrum where RD-driven water flip-back occurs only at the end of the mixing time during the delay τrd. (c) TOCSY pulse sequence and corresponding spectrum with RD-driven water flip-back at the end of the mixing time. NMR experiments were performed at 800 MHz using a cryoprobe and a sample of 0.5 mM solution of ubiquitin fused to a 15-residue peptide in 90%H2O/10%D2O at pH 7.0 and 25 °C. The NOESY and TOCSY mixing times were 60 ms. Refer to [84] for additional details. Pulses sequence and spectral data are adopted from [84] (reproduced with permission).