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. 2013 Apr 25;3(4):1080–1092. doi: 10.1016/j.celrep.2013.03.012

Figure 3.

Figure 3

Mapping CoTC Terminator Elements at Endogenous Gene Loci

(A) RT-PCR analysis of CCNB1 3′ flanking region transcripts using primer pairs indicated (blue arrows) in the diagram below the data panel. Lanes 1–5, control PCR amplification of genomic DNA. Lanes 6–10 (+RTase) PCR amplification of reverse transcribed CCNB1 3′ flanking region transcripts. Lanes 11–15, control PCR amplification of −RTase samples. The diagram below the data panel shows the CCNB1 3′ UTR (gray box), poly(A) site (green arrowhead), and putative CoTC terminator element (PCTE, red bar).

(B) RT-PCR analysis of PTCH2, THOC2, AKIRIN1, and WDR13 3′ flanking region transcripts. Sample lanes and diagram notation are as in (A). ∗In PTCH2 lane 8, indicates nonspecific RT-PCR products of unknown origin. Control PCR amplification (−RTase, lanes 11–15) confirms the absence of contaminating DNA.

See also Figure S1.