Figure 7.

Redistribution of TJ proteins from membrane to high-density fractions in the small intestine of pups submitted to the NEC protocol for 24 hours. Subcellular fractions from small intestines of DF controls and stressed pups were separated by centrifugation on a discontinuous sugar density gradient and submitted to Western blotting and quantification of claudin proteins and specific subcellular markers. Protein disulfide isomerase (PDI) was used as an endoplasmic reticulum marker and caveolin 1 as a lipid raft marker. Results are representative of two independent experiments, run in triplicate.