Figure 6.

miR-101 suppresses tube formation of human umbilical vein endothelial cells in vitro. Human umbilical vein endothelial cells (3 × 10⁴) in 100 μL 200PRF medium were mixed with 400 μL conditional medium from indicated cells and plated in 24-well plates coated with growth factor–reduced Matrigel. The cells were incubated for 3 to 5 hours and stained with Calcein AM before immunofluorescence microscopy. A: Representative images of human umbilical vein endothelial cell tube formation. B: Upper panel: Normalized tube length of cells with indicated transduction or treatment. Lower panel: Branching points of cells with indicated transduction or treatment. The tube length and branching point of each scramble control was set as 100%. The data are expressed as means ± SEM from three independent experiments. L/control, control lentivirus; L/miR101, miR-101-1 lentivirus. ^∗P < 0.05, ^∗∗P < 0.01.