Figure 5.

The mSplunc1 expression is upregulated in the mouse lung after an induced respiratory infection. Wild-type C3HeB/FeJ mice were infected with 1000 CFUs/mouse lung K. pneumoniae and sacrificed at 6, 24, and 48 hours after infection. Murine Splunc1 mRNA expression was quantified in lung homogenates by quantitative PCR and determined by the ΔΔCt method using mouse glucuronidase-β RNA as a control. All data are expressed as fold induction compared to 0 hour time point. Results are means ± SEM from three separate experiments (n = 6 mice for each group). *P < 0.05.