(A) Panc1 cells were transfected for 72 h with siRNA for CPI-17 (right lane) or non-targeting control (left lane, Ctl), and subjected to immunoblotting using antibodies against proteins indicated. P denotes phosphorylated protein. MLN; merlin, ERM; ezrin/radixin/moesin, H3; histone H3. (B) Effects of CPI-17 knockdown on cell proliferation. After 72-h knockdown, cells were stained with hematoxylin, and the number of cells was counted under microscope. The cell number before the knockdown was set as 1.0. n=3, * p<0.05 (Student’s t-test).