Table 1.
Thermal denaturation temperatures (Tm) and fluorescence quantum yields (ΦF) of singly and doubly modified Glowing LNA probes in different backbone contexts and their duplexes with complementary DNA/RNA (1X, 5'-GCA T(U)A37 CAC; 2X, 5'-GCA 37A37 CAC). (Reproduced with permission from ref. 91. Copyright 2010 American Chemical Society).
|
Tm (△Tm/mod)/°C |
quantum yield (ΦF) |
||||
|---|---|---|---|---|---|
| probe | +DNA | +RNA | SSP | +DNA | +RNA |
| DNA 1X | 32.5 (+3.0) | 33.0 (+6.0) | 0.50 | 0.79 | 0.91 |
| DNA 2X | 40.5 (+5.5) | 41.5 (+7.5) | 0.27 | 0.81 | 0.70 |
| PS-DNA 1X | 24.0 (+3.5) | 26.0 (+7.5) | 0.50 | 0.64 | 0.66 |
| PS-DNA 2X | 32.0 (+6.0) | 35.0 (+8.5) | 0.20 | 0.61 | 0.62 |
| RNA 1X | 31.5 (+4.5) | 42.5 (+4.5) | 0.84 | 0.89 | 0.93 |
| RNA 2X | 38.0 (+5.5) | 46.5 (+4.0) | 0.16 | 0.85 | 0.89 |
| O2'-Me 1X | 31.0 (+5.0) | 45.0 (+4.5) | 1.00 | 0.97 | 0.98 |
| O2'-Me 2X | 20.0 (−3.0) | 46.0 (+3.0) | 0.14 | 0.68 | 0.86 |
| LNA 1X | 54.5 (−3.5) | 64.0 (−5.0) | 0.95 | 0.90 | 0.89 |
| LNA 2X | 49.0 (−4.5) | 60.5 (−4.5) | 0.72 | 0.92 | 0.84 |