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IFN levels increase in a time- and replication-dependent fashion. IFN levels were assayed from infected cell culture supernatant. 5/3Cox2CRAdΔE3ADP-IFN shows a robust production of IFN despite its control of replication by Cox2. As expected the 5/3WtΔE3ADPIFN not under replication control by Cox2 attains greater levels of IFN production but is used for proof of principle only and is not suitable for use in vivo.
