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. Author manuscript; available in PMC: 2013 May 6.
Published in final edited form as: Biochim Biophys Acta. 2011 Jul 21;1814(12):1955–1963. doi: 10.1016/j.bbapap.2011.07.012

Figure 4. Km and kcat values for hydrolysis of FXIII V34X peptides by thrombin mutants.

Figure 4

(A) Visual comparison of the Km values for FXIII (28–41) V34 (white), V34L (dotted), V34P (gray), and V34F (dark gray) AP in the presence of the thrombin mutants W60dA, Y60aA, W215A, E217A, and WE. (B) Visual comparison of the kcat values for FXIII (28–41) V34 (white), V34L (dotted), V34P (gray), and V34F (dark gray) AP in the presence of the thrombin mutants W60dA, Y60aA, W215A, E217A, and WE. An HPLC assay described in Experimental Procedures was used to obtain data to calculate these individual kinetic parameters. The results shown represent averages from at least three independent experiments.