| 10 x buffer | 2 μl |
| PCR products (0.5-1 μg) | 1.5 μl |
| ATP (75mM) | 2 μl |
| GTP (75mM) | 2 μl |
| CTP (75mM) | 1.5 μl |
| UTP (75mM) | 1.5 μl |
| Biotin-11-CTP (10mM) | 3.75 μl |
| Biotin-16-UTP (10mM) | 3.75 μl |
| T7 RNA polymerase | 2 μl |
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