Skip to main content
. Author manuscript; available in PMC: 2013 May 6.
Published in final edited form as: RNA Biol. 2009 Nov-Dec;6(5):575–583. doi: 10.4161/rna.6.5.10079

Figure 3.

Figure 3

miR-125a suppression of cell proliferation is partially rescued by HuR. MCF-7 cells were mock transfected or transfected with miR-125a precursor for 24 h followed by transfection of pcDNA3.1 (mock+vec, 125a+vec) or pcDNA3.1mycHuR (mock+HuR, 125a+HuR) for 48 h. A, Cell proliferation was assessed by immunofluorescence analysis of Ki-67, a marker of dividing cells. Representative fluorescence micrographs are shown. Top panels, Ki-67 was visualized with Alexa Fluor 488 conjugated secondary antibody (green). Middle panels, nuclei were stained with DAPI (blue). Bottom panels, merge of Ki-67 and DAPI channels. Magnification, 200×. Percentage of Ki-67+ cells was calculated by counting 500 cells for each condition. B, Cell number in each condition was assessed by cell counting and expressed as percent of mock+vec transfected cells. The data shown are representative (micrographs) or means of three independent experiments. ** p< 0.01 vs. mock+vec, # p< 0.05 vs. 125a+vec.