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Neomycin stabilizes an intermediate conformation of the ribosome. (a) Illustration showing the ribosome labeling strategy used for the single-molecule FRET experiments. (b,c) Single-molecule FRET trajectories, as shown in Supplementary Figure 2, summed into population FRET histograms to reveal the population behaviors of Cy3-labeled S13 (N terminus) and Cy5-labeled L1 (T202C) ribosome complexes bearing deacylated tRNAfMet (b) or deacylated tRNAPhe (c) in the P site.
