Fig. 3.

DsRed normalized mRNA abundance and genome copy number from uninsulated and insulated transgenes inserted randomly into the An. stephensi genome. (A) Fifteen uninsulated, control transgenic lines (C-1 to C-15), and (B) 20 insulated transgenic lines (G-1 to G-20) were generated and analyzed for DsRed mRNA abundance levels. DsRed mRNA accumulation was quantified by qRT-PCR in two different mosquito generations, G₂ (dark-color bars) and G₅ (light-color bars). Statistically significant differences in mRNA abundance between generations are marked with an asterisk (*). The number of DsRed genes present in G₂ mosquitoes was determined by qPCR and is indicated below each transgenic line. DsRed mRNA levels were quantified by qRT-PCT and normalized using ribosomal gene S7 transcript abundance as a reference.