Table 2.

Validation of RNA-sequencing using relative quantitative PCR

Gene name	Location	RNA-sequencing, metafemale/female	Relative quantitative PCR	Max	Min
Ssp3	2L	0.67	0.602	0.755	0.48
Jet	2L	1	1.179	1.325	1.048
Ssrp	2R	0.67	0.524	0.576	0.477
Magu	2R	1	1.132	1.273	1.007
Lysp	3L	0.167	0.114	0.136	0.097
CG16758	3L	0.512	0.259	0.304	0.221
Oxt	3L	1	1.043	1.311	0.829
Kap-alpha1	3L	1	0.942	1.028	0.863
Ac76E	3L	1.98	1.34	1.607	1.117
Sp7	3R	0.37	0.214	0.252	0.181
Gish	3R	0.67	0.595	0.655	0.541
Nup358	3R	0.67	0.693	0.791	0.607
Rpl3	3R	1.18	1.046	1.23	0.89
Sw	X	1.5	1.341	1.435	1.253
Out	X	1.5	1.313	1.443	1.196
Ag5r2	X	1.5	1.571	1.675	1.473
TLK	X	1	0.932	1.01	0.86
Ing3	X	1	0.954	1.204	0.756
CG9577	X	1	1.028	1.192	0.886
Myb	X	1	0.908	1.08	0.763
Eo-ry	X	1.4	1.475	1.719	1.265
fog	X	0.96	0.928	1.126	0.764
Karl	X	0.829	0.574	0.671	0.492
CG15771	X	1.76	1.529	1.924	1.215

Ratios of selected gene expression when metafemales (XXX; AA) were compared with normal females (XX; AA) are shown. Three biological and technical replicates were applied to each genotype in RT-PCR. The relative quantification (RQ) for each pair of primers was measured based on the ΔCt analysis according to the instructions from the manufacturer (7300 Real-time PCR system, sequence detection software version 1.3.1; Applied Biosystems). The data were analyzed with calculation of the 95% confidence interval. The average RQ of each gene with its maximum RQ (Max) and minimal RQ (Min) is shown.