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. 2013 Apr 15;110(18):7288–7293. doi: 10.1073/pnas.1304285110

Fig. 2.

Fig. 2.

Energy production by OXPHOS is essential for hepatocyte polarization. (A) Immunofluorescence analysis of occludin (green) and ABCB1 (red) in day 1 cells treated with 0.1 μg/mL oligomycin, 1,600 nM FCCP, or 100 mM 2-DG for 24 h. Merged projection images are from Z-serials of confocal images. (B) Canalicular lengths from three individual experiments. *P < 0.05; ***P < 0.001 compared with control. (C) Percentage of ATP after treatment of day 1 cells with 10 μg/mL oligomycin, 1,600 nM FCCP, or 100 mM 2-DG for 4 h, normalized to control. ***P < 0.001 compared with control. Data are from three individual experiments. (D) Immunofluorescence analysis of mitochondrial outer membrane protein Tom20 in day 1 cells treated with 10 μg/mL oligomycin, 1,600 nM FCCP, or 100 mM 2-DG for 24 h. Insets show zoomed-in views.