Fig. 2.

Descendants of embryonically labeled Axin2^CreERT2 cells become functional adult SVZ and DG stem cells. Representative images of E8.5 or E12.5 tamoxifen-induced Axin2^CreERT2/+; R26R^mTmG/+ embryos. (A–C) E8.5–P21; (D–K) E12.5–P56. (A, D, and E) Lateral ventricle; (B and F) rostral migratory stream; (C and G) olfactory bulb; and (H and K) hippocampus. (A) E8.5-labeled Axin2⁺ RGCs produce GFP⁺ progeny with morphologies consistent with ependymal and subependymal cells, (B) migratory neuroblasts, and (C) olfactory neurons. (D) Progeny of Axin2⁺ cells labeled at E12.5 also generate GFP⁺ subependymal cells, some of which also stain for GFAP (red, E). (F) GFP⁺ neuroblasts and (G) olfactory neurons are also present. (H) Progeny of Axin2⁺ cells traced from E12.5–P56 make significant contributions to multiple structures in the hippocampus. (I) Some Axin2⁺ descendants are radial cells that coexpress GFAP; Inset shows GFAP only. (J) Clusters of GFP⁺ Dcx⁺ cells are also present (Inset shows Dcx only), as well as many GFP⁺ calbindin⁺ neurons (Inset shows calbindin only) (K). (Scale bar, 50 μm.) CP, choroid plexus. Boxed region in D is magnified in E. Arrows point to cells of interest and double positive cells; dashed lines in D and E mark the edge of the ventricle. DAPI staining is shown in blue, GFP staining in green, and all other markers in red.