Fig. 3.

Descendants of embryonically labeled Axin2^CreERT2 cells are regionally and developmentally restricted adult SVZ stem cells. Coronal sections of Axin2^CreERT2/+; R26R^mTmG/+ embryos traced from E12.5–P56. (A and B) Lateral ventricle; (C–F) olfactory bulb. (A and B) E12.5–P56 traces reveal that the progeny of Axin2⁺ cells are regionalized postnatally and are only found on the medial and dorsal walls (Inset) of the lateral ventricle. (C–F) In the olfactory bulb, adult SVZ descendants of E12.5 Axin2⁺ cells generate (C and E) calretinin⁺ GFP⁺, and (D) tyrosine hydroxylase⁺ GFP⁺ olfactory neurons, but not (F) calbindin⁺ GFP⁺ olfactory neurons. CP, choroid plexus; L, lateral; M, medial. Boxed region in A is magnified in B; Insets in C–E magnify double positive cells. Arrowheads point to cells of interest. GFP is shown in green, DAPI is shown in blue, and all other markers are shown in red. (Scale bar, 50 μm.)