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. 2013 Apr 15;110(18):7506–7511. doi: 10.1073/pnas.1301251110

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Fig. 1. — Schematic representation of the apicoplast purification procedure. Free parasites are released from host RBCs via saponin lysis. An organelle-enriched fraction (organelles) is obtained by hypotonic shock and low-speed centrifugation. Free apicoplasts are retrieved from the mixed organelles with magnetic beads coated in antibodies directed against the apicoplast outer membrane bait protein PfoTPT-HA using a magnetic collector. A, apicoplast; Cyt, parasite cytoplasm; FV, food vacuole; Mt, mitochondrion; PfoTPT-HA, apicoplast outer membrane triose phosphate transporter expressing a HA tag facing the cytosol; PM, parasite plasma membrane; PV, parasitophorous vacuole; PVM, parasitophorous vacuole membrane; RBC Cyt, RBC cytoplasm; RBC PM, RBC plasma membrane.