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. 2013 Apr 15;110(18):7506–7511. doi: 10.1073/pnas.1301251110

Fig. 3.

Fig. 3.

Lipidomic analysis of whole parasites and apicoplasts by GC-MS, LC-MS, and LC-MS/MS. (A) Fatty acid composition obtained by GC-MS analysis (mol%, n = 4 independent experiments; mean estimate ± SD). (B) Major polar lipid composition quantified by multiple reaction monitoring acquired by LC-MS/MS (mol%). (C) PI composition by LC-MS (mol% within its class). (D) DAG composition by LC-MS (mol% within its class). (E) Cer content by LC-MS analysis (arbitrary unit: area/μM of total lipid, apicoplast fraction content is presented relative to total content in whole parasite fraction). n = 4 independent experiments; mean estimate ± SD (F and G). Labeling of C14:0 (myristate) with [U-13C]-glucose in fatty acid rich medium (G) and in medium with minimal fatty acid (C16:0, C18:1). Only regions of the mass spectra of the fatty acid methyl ester showing M+ ions are shown. m/z 242 represents monoisotopic mass of myristylmethylester, whereas m/z 256 represents fully labeled species. FA, fatty acid; LPC, lysophosphatidylcholines; PG, phosphatidylglycerols; PS, phosphatidylserines.