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. 2013 Apr 9;110(18):E1676–E1684. doi: 10.1073/pnas.1217712110

Fig. 3.

Fig. 3.

A subset of the IGMs forms stage I gametocytes. (A) Pfmdv1/peg3 is a gametocyte-specific gene expressed in all gametocyte stages (13). Immunofluorescence using antisera against Pfmdv1/peg3 identified the protein in stage I gametocytes from IGMs 2E2, 1C5, 2B4, 1G1, 2F6, and 1B5. (B) Pfmdv1/peg3 staining also reveals that IGM 1B5 is able to produce what appears to be stage IIA gametocytes but not stage IIB or later gametocytes (8). None of the IGMs produced gametocytes that mature beyond stage I or IIA. (C) Under the same experimental conditions, the antibody consistently identified the protein in stages I–V gametocytes from the parental line.