Table 1.
piggyBac screen identifies 16 insertion events that putatively disrupt gametocytogenesis
| Insertion number | IGM | Accession | Annotation | Chromosome | Insertion position | mRNA abundance | ORF size (bp) |
| 1 | 2F11 | PF14_0532 | LCCL domain-containing (PfCCP2) | 14 | ORF | Decreased | 5,115 |
| 2 | 2H3 | PFE1370w | Putative Hsp70-interacting protein (PfHip) | 5 | 5′ of ORF | Decreased | 1,742 |
| 3 | 2A2 | PFC0200w | 60S ribosomal protein (Rpl36a) | 3 | 5′ of ORF | Absent | 549 |
| 4 | 2A11 | PFD0800c | Repressor of RNA polymerase III (MAF1) | 4 | 5′ of ORF | Decreased | 1,170 |
| 5 | 2H8 | PF14_0097 | CDP-DAG synthase | 14 | 5′ of ORF | Absent | 2,004 |
| 6 | 2A9, 2H2 | PFL1770c | p25a/TPPP | 12 | 5′ of ORF | Absent | 580 |
| 7 | 2B4 | PF11_0167 | ZDHHC-domain containing Palmitolyation factor | 11 | 5′ of ORF | Absent | 2,040 |
| 8 | 2D3 | PFL2550w | Type IV HSP-interacting (PfGECO) | 12 | 5′ of ORF | Decreased | 1,579 |
| 9 | 2E4, 2F12 | PF13_0097 | AP2 transcription factor | 13 | ORF | Decreased | 8,019 |
| 10 | 2G2, 2G11 | PFI1215w | SF3A3 | 9 | 5′ of ORF | Absent | 1,770 |
| 11 | 2F4 | PFE1615c | Small PEXEL-containing hypothetical protein | 5 | ORF | Absent | 699 |
| 12 | 2E2 | MAL7P1.171 | Large PEXEL-containing hypothetical protein | 7 | 5′ of ORF | Decreased | 6,439 |
| 13 | 2F6 | PFI0980w | Long-chain fatty acid elongase enzyme (ELO3) | 9 | ORF | Absent | 1,929 |
| 14 | 1B5 | PF07_0055 | AAT-1/C. reinhardtii flagellar-associated protein 91 component of eukaryotic flagellar regulatory complex | 7 | 5′ of ORF | Absent | 2,049 |
| 15 | 1C5 | PF14_0595 | Calponin homology domain protein | 14 | ORF | Absent | 4,962 |
Clones with single piggyBac insertions were named IGM. Inverse PCR was used to identify piggyBac insertion sites (Table S1). A total of 16 insertion sites was identified among 22 IGMs with single piggyBac insertions, because some of the independently isolated IGMs had insertions at identical sites (Tables S1 and S2). In cases where the same disruption site was identified in multiple IGMs, the IGM used for additional characterization is indicated in italics. Sequence analysis revealed that insertions occurred in nine different chromosomes, indicating genome-wide distribution of piggyBac insertion events. mRNA abundance was estimated by semiquantitative RT-PCR (Fig. S1). AP2, Apicomplexan Apetala 2; HSP, heat shock protein; LCCL, Limulus clotting factor C; PEXEL, plasmodium export element; ZDHHC, zinc-finger aspartate-histidine-histidine-cysteine.