Skip to main content
. 2013 Apr 15;110(18):7395–7400. doi: 10.1073/pnas.1221608110

Fig. 4.

Fig. 4.

Knockout or knockdown of β-arrestin1 reduces the production of TH17-associated cytokines. (A) ELISA of CII-specific IgG1, IgG2b, or IgG3 in the serum of Arrb1−/− mice and WT littermates at day 0 and day 35 after initial immunization (n = 7 per group). (B) Flow cytometry of intracellular IL-17, IFN-γ, or Foxp3 in gated CD4+ T cells from the spleen and draining lymph node (DLN) of CII-immunized Arrb1−/− mice and WT littermates (n = 8 per group). (C) Concentration of IL-17 or IFN-γ in the supernatants of cell cultures challenged with CII (100 μg/mL) for 4 d (n = 6 per group). (D) Chemotaxis assay of DLN cells or CD4+ T cells from CII-immunized Arrb1−/− mice and WT littermates (n = 5 per group). (E and F) Immunoblot of GFP or β-arrestin1 (E) and expression of various cytokines (F) in the joints of CII-immunized mice intraarticularly injected with lentivirus expressing GFP and shRNA for Arrb1 (Lenti-shArrb1) or GFP and control shRNA (Lenti-control) (n = 5 per group). (G–I) Flow cytometry of GFP (G) and expression of various genes (H and I) in CD4+CCR6+ T cells from mice with CIA transduced with Lenti-shArrb1 or Lenti-control.