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. 2013 Apr 15;110(18):7395–7400. doi: 10.1073/pnas.1221608110

Fig. 5.

Fig. 5.

Depletion of β-arrestin1 impairs TH17 cell differentiation in vitro. (A) Expression of Arrb1 in TH0, TH1, TH2, TH17, or inducible Treg (iTreg)-polarized cells from C57BL/6 mice. (B) Quantitative RT-PCR of Arrb1 mRNA in naïve CD4+ T cells or TH17-polarized cells in the presence of DMSO or Digoxin (10 μM). (C) Flow cytometry of naïve CD4+ T cells from Arrb1−/− mice and WT littermates cultured for 4 d under TH1, TH2, TH17, or iTreg-polarizing conditions. Percentage of each subset is shown. (D) Production of IL-17 in the supernatants of TH17-polarized cells. (E) Expression of TH17-associated genes in naïve CD4+ T cells from Arrb1−/− mice and WT littermates cultured for 2 d under TH17-polarizing conditions. (F) Flow cytometry of naïve CD4+ T cells labeled with carboxyfluorescein succinimidyl ester (CFSE) from Arrb1−/− mice and WT littermates cultured for 4 d under TH17-polarizing conditions. (G) Naïve CD4+ T cells were cultured under TH17-polarizing conditions, and live cells were identified by propidium iodide exclusion.