Fig. 2.

Structural characterization of [2Fe-2S]-CIAPIN1-single. (A) ¹H-¹⁵N IR-HSQC-AP spectrum, optimized to detect fast relaxing ¹H resonances, showing 13 backbone NH resonances of the [2Fe-2S]-CIAPIN1-single, 10 of which (in black) were completely lost in the standard diamagnetic ¹H-¹⁵N HSQC experiment (the three residues also detected in the diamagnetic ¹H-¹⁵N HSQC are shown in gray. (B) Representative model of the CIAPIN1 domain of anamorsin derived from a molecular dynamics simulation of 100 ns. The [2Fe-2S] cluster bound to the CX₈CX₂CXC motif is shown; the four cysteines coordinating the [2Fe-2S] cluster and the four cysteines of the CX₂CX₇CX₂C motif are shown.