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. 2013 Mar 27;14(4):6920–6959. doi: 10.3390/ijms14046920

Figure 13.

Figure 13

Effect of human MICAL-MO on actin polymerization state as determined fluorimetrically. (A) The polymerization state of pyrene-labelled G-actin (4.1 μM) in the presence or absence of NADPH (100 μM, N) and human MICAL-MO (660 nM, M) was monitored fluorimetrically in a Cary Eclipse (Varian) fluorimeter. The polymerization was induced by addition of 1/10 volume of polymerization buffer (P, 50 mM Tris/HCl, pH 7.5, 500 mM KCl, 20 mM MgCl2, 5 mM DTT, 10 mM ATP) at the indicated time to the solution in G-buffer (5 mM Tris/HCl, pH 8.0, 0.2 mM CaCl2, 0.2 mM ATP, 1 mM DTT); (B) Fluorescence-monitored polymerization and depolymerization of actin (2.3 μM) in the presence of NADPH (100 μM, N) and human MICAL-MO (660 nM, M) added at the indicated times. In the panels the traces have been offset for graphical purposes. Reprinted from [70] with permission from Elsevier.