Table 2.
Summary of the kinetic properties of the NADPH oxidase reaction of human MICAL-MO. Assays were carried out at 25 °C in the presence of the indicated NAD(P)H concentration ranges and additions in 20 mM Hepes/KOH buffer, pH 7.0. The last line reports the apparent Kd for NADPH [86] and kred calculated from an anaerobic stopped-flow experiment in which MICAL-MO was reacted with varying concentrations of NADPH. Data are from [70].
| NADPH, μM | NADH, μM | NaCl, M | Glycerol, % | KM, μM | kcat, s−1 | kcat/KM, s−1 mM−1 |
|---|---|---|---|---|---|---|
| 4–160 | 28 ± 2 | 4.0 ± 0.1 | 143 ± 11 | |||
| 40–650 | 0.1 | 499 ± 28 | 2.6 ± 0.1 | 5.2 ± 0.4 | ||
| 20–200 | 28 ± 3 | 4.4 ± 0.1 | 157 ± 17 | |||
| 80–670 | 580 ± 24 | 0.28 ± 0.01 | 0.48 ± 0.03 | |||
| 10–300 | 26 ± 4 | 3.9 ± 0.1 | 150 ± 23 | |||
| 10–300 | 10 | 93 ± 11 | 2.9 ± 0.1 | 31.2 ± 4 | ||
| (reductive half reaction) | 10 | 56 ± 7.3 | 3 ± 0.1 |