Figure 5.

Proline content in Col-0, wnk8 and WNK8-OE under salt and osmotic stress conditions. Three-day-old seedlings grown in standard 1/2 MS media were transferred to soil and further cultivated for 20 days and then treated with 150 mM NaCl or 300 mM sorbitol via irrigation once every three days. After 15 days, the proline content was measured. Data are the means ± SE from three independent experiments, and each experiment included 20 uniform seedlings tested. Asterisks on bars indicate significant differences in contrast to the wild-type under the same treatment (Student’s t-test, p < 0.05).