Table 3.

Half-maximal inhibition concentrations (IC₅₀-values) of various compounds for the inactivation of alcohol dehydrogenase (26 nM) by peroxynitrite (20 μM).

Scavenger	IC50 (μM)	Scavenger	IC50 (μM)
Glutathione	31 ± 2.5	Methionine	185 ± 7
Ascorbate	***	Uric acid	400/26 ± 2 *
2,6-Dithiopurine	40 ± 5 **	2,6-Dithiopyrimidine	45 ± 8 **
Ebselen	***	3,7-Dimethyluric acid	400/42 ± 1.5 *
Se-methionine	15 ± 1	Tryptophan	400/35 ± 3 *
Cysteine	18 ± 3	Tyrosine	420 ± 30

^*highest concentration of scavenger/percentage of preserved ADH-activity at this concentration;

^**scavenger itself reduced ADH-activity by 20% when used at 50 μM;

^***strong inhibition of ADH by ebselen and probably a product from the peroxynitrite-ascorbate reaction.