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. 2013 Apr 12;14(4):8073–8092. doi: 10.3390/ijms14048073

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© 2013 by the authors; licensee MDPI, Basel, Switzerland

This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution license (http://creativecommons.org/licenses/by/3.0/).

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The effect of chemical redox compounds on enzyme activity. (a) np-Ga3PDHase; (b) Ga3PDHase. Each enzyme was incubated as detailed under Experimental Section. When indicated by the arrows it was added 1 mM diamide and then 10 mM dithiotreitol (DTT). At the specified time aliquots were withdrawn and assayed for the respective enzymatic activity. At the beginning of oxidation assays both enzymes were fully active (100%): (a) 21 U/mg and (b) 38 U/mg. Error bars represent the values deviation in three independent assays.

The effect of chemical redox compounds on enzyme activity. (a) np-Ga3PDHase; (b) Ga3PDHase. Each enzyme was incubated as detailed under Experimental Section. When indicated by the arrows it was added 1 mM diamide and then 10 mM dithiotreitol (DTT). At the specified time aliquots were withdrawn and assayed for the respective enzymatic activity. At the beginning of oxidation assays both enzymes were fully active (100%): (a) 21 U/mg and (b) 38 U/mg. Error bars represent the values deviation in three independent assays.