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. 2013 Apr 16;14(4):8291–8305. doi: 10.3390/ijms14048291

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© 2013 by the authors; licensee MDPI, Basel, Switzerland

This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution license (http://creativecommons.org/licenses/by/3.0/).

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Levels of bFGF, VEGF and PEDF proteins estimated by western blot analysis. The α-tubulin band was used as the control for normalization. (A) The bFGF bands (molecular weight, approximately 18 kDa) produced with 1 μg of the p-bFGF-encoding plasmid after day 6; (B) Visualization of the PEDF bands (molecular weight, approximately 50 kDa) produced with 0 μg, 0.1 μg, 1 μg, and 10 μg of PEDF-encoding plasmid. This band was also noted in the 0 μg PEDF group due to endogenous PEDF. The inhibition of VEGF expression was more significant in the 10 μg + 10 μg bevacizumab + p-PEDF-SAINT-18 group; (C) On day 60 after administration, the PEDF and VEGF levels within the corneal and subconjunctival substantia propria were determined.