Figure 4.

Removal of SUMOylation reduces ATF3-mediated cellular proliferation and colony formation in PC3 cells. (A) Cell numbers were determined in indicated time after plating of WT ATF3-expressed or K42R ATF3-expressed cells by cell counting assay. On days 3 and 4, cells were also photographed. For each test, p values of <0.05 and <0.001 were considered significant (*) and very significant (**), respectively; (B) DeSUMOylation reduced ATF-enhanced the colony formation of PC3 and DU145 cells, as shown by representative dishes of colony formation. All experiments were repeated at least three times with the same results. The graph shows the mean results and S.D. of three independent experiments.