Figure 1.
Comparison of peptide helical structure content in four peptides at 0–99% TFE concentration. The titration curves demonstrate that for three peptides their inherent structure begins stabilizing at low TFE concentrations and reach half maximal structures around 20% TFE. Nonlinear regressions of mean residue molar ellipticity (MRME) values for Cx32-NT, -CL1, -CL2, and CT2 shows half-maximal values (EC50) at 20.1, 21.3, 18.1, and 29.5% of TFE. Note that Cx32-CT2 peptide demonstrates little structure even at high TFE. The dotted line marks 30% TFE.