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. 2013 May 6;8(5):e62507. doi: 10.1371/journal.pone.0062507

Figure 2. Short term removal of FGF2 at 5% O2 alters hESC metabolism and promotes O2 consumption.

Figure 2

Removal of FGF2 for 16 hours from Hues7 hESCs cultured at 5% O2 (5% O2– FGF2) resulted in a reduction of glucose consumption and lactate production, whereas pyruvate consumption dramatically increased (A). Shef3 hESCs cultured at 5% O2– FGF2 displayed a significant reduction in lactate production (B). **P<0.01, ***P<0.001 significantly different to 5% O2+FGF2 (n = 10–18). Hues7 hESCs cultured at 5% O2 consumed less O2 than when FGF2 was removed for 16 hours (C). hESCs maintained at 20% O2 consumed the greatest amount of O2. Bars with the same superscript are significantly different; a, b, P<0.001, c, P<0.05 (n = 7–8).