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. 2013 May 6;8(5):e60664. doi: 10.1371/journal.pone.0060664

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© 2013 Siddique et al

This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.

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(A–B) While PrEC replicated for 5 passages and entered into senescence, BMI1-rich counterparts replicated and avoided senescence upto 8th passages. (A) Inset 400X of magnified areas show senescent morphology features i.e. globular shape and (B) indicate staining for ß-galactosidase. (C) Scattered Plot for qPCR array. The dots indicate gene expression on a log-scale representing the change in BMI1 silenced-LNCaP cells. Fold change (2∧- Delta Ct) is the normalized expression (2∧-Delta Ct) in the BMI-silenced cells divided by the normalized expression of Control. (D and E) Immunoblots represents the effect of BMI1-silencing and BMI1-overexpression on the expression of Cyclin-D1, BCL2 and p16 proteins in cells. The equal loading of protein was confirmed by ß-actin.

Inline graphic — (A–B) While PrEC replicated for 5 passages and entered into senescence, BMI1-rich counterparts replicated and avoided senescence upto 8th passages. (A) Inset 400X of magnified areas show senescent morphology features i.e. globular shape and (B) indicate staining for ß-galactosidase. (C) Scattered Plot for qPCR array. The dots indicate gene expression on a log-scale representing the change in BMI1 silenced-LNCaP cells. Fold change (2∧- Delta Ct) is the normalized expression (2∧-Delta Ct) in the BMI-silenced cells divided by the normalized expression of Control. (D and E) Immunoblots represents the effect of BMI1-silencing and BMI1-overexpression on the expression of Cyclin-D1, BCL2 and p16 proteins in cells. The equal loading of protein was confirmed by ß-actin.