Table 1. Oligonucleotides for quantification and detection of HAdV.
| Primer | Oligonucleotide sequence (5′–3′) |
| TaqMan assaysa | |
| AdVC E1A for | GACGGCCCCCGAAGATC |
| AdVC E1A probe | CGAGGAGGCGGTTTCGCAGA |
| AdVC E1A rev | TCCTGCACCGCCAACATT |
| AdV5 E3del for | CTACGGGCTATTCTAATTCAGGTTTC |
| AdV5 E3del probe | TGCGCTGTTGCTCGGCCG |
| AdV5 E3del rev | CCATGTCTTGGAGCTCTTGATTC |
| Nested PCR for HAdV typingb | |
| AdVC E1 for | ATACCCGGTGAGTTCCTCAAG |
| AdVC E1 rev | CGCCATTTTAGGACGGCGG |
| AdVC E1 for n | ACTCTTGAGTGCCAGCG |
| AdVC E1 rev n | GGACGGCGGGTAGGTC |
a
The probe sequences indicated are TagMan probes labeled with 6-carboxyfluorescein on the 5′ end and with 6′-corboxytetramethylrhodamine on the 3′ end; b AdVC E1 for n and AdVC E1 rev n are primers for the second round of the nested PCR.