Table 1. Large-scale Orthopteran transcriptome resources to date.
Locusta migratoria 1 | Laupala kohalensis 2 | Schistocerca gregaria 3 | Locusta migratoria 4 | Gryllus bimaculatus 5 | |
Orthopteran Suborder | Caelifera | Ensifera | Caelifera | Caelifera | Ensifera |
Superfamily | Acridoidea | Grylloidea | Acridoidea | Acridoidea | Grylloidea |
Family | Acrididae | Gryllidae | Acrididae | Acrididae | Gryllidae |
Sequencing Platform | Sanger | Sanger | Sanger | Illumina | 454 Titanium |
Tissue Source(s) | L56 | L5–L8 CNS | L3–L5 & adult CNS | Mainly L4 | Ovaries & embryos |
Normalized Library | No | Yes | Yes | No | No |
# Raw Reads | 76,012 | 14,502 | nd | 447,718,464 | 4,248,346 |
# Reads Used in Assembly | 45,449 | 14,377 | 34,672 | nd | 4,216,721 |
# bp Used in Assembly | 21,760,812 | 10,121,408 | nd | nd | 1,449,059,795 |
% Raw Reads Assembled | 59.79% | 99.14% | nd | nd | 99.26% |
# Contigs or Clusters | 4,550 | 2,575 | 4,785 | 72,977 | 43,321 |
N50 7 or Mean Contig Length (bp) | 471 | 935 | 750 | 2,275 | 2,133 |
# Singletons or # Single ESTs 8 | 7,611 | 6,032 | 7,924 | nd | 120,805 |
% Singletons (of assembled reads) | 16.75% | 41.96% | 22.85% | nd | 2.86% |
# Total Assembly Products | 12,161 | 8,607 | 12,709 | 72,977 | 142,317 |
# Unigenes or # Unique BLAST 9 Hits to nr | 12,616 | 8,607 | 12,709 | 11,490 | 19,874 |
Data from [75].
Data from [76].
Data from [72].
Data from this report.
“N50” refers to isotig N50 from the G. bimaculatus de novo transcriptome assembly; mean contig length is shown for all other orthopteran transcriptome resources in this table.
# singletons are shown for the G. bimaculatus de novo transcriptome assembly; # single ESTs (not incorporated into contigs) are shown for all other orthopteran transcriptome resources in this table.
# unique BLAST hits against nr are shown for the G. bimaculatus de novo transcriptome assembly; # unigenes are shown for all other orthopteran transcriptome resources in this table.