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. 2013 May 6;8(5):e61479. doi: 10.1371/journal.pone.0061479

Table 1. Large-scale Orthopteran transcriptome resources to date.

Locusta migratoria 1 Laupala kohalensis 2 Schistocerca gregaria 3 Locusta migratoria 4 Gryllus bimaculatus 5
Orthopteran Suborder Caelifera Ensifera Caelifera Caelifera Ensifera
Superfamily Acridoidea Grylloidea Acridoidea Acridoidea Grylloidea
Family Acrididae Gryllidae Acrididae Acrididae Gryllidae
Sequencing Platform Sanger Sanger Sanger Illumina 454 Titanium
Tissue Source(s) L56 L5–L8 CNS L3–L5 & adult CNS Mainly L4 Ovaries & embryos
Normalized Library No Yes Yes No No
# Raw Reads 76,012 14,502 nd 447,718,464 4,248,346
# Reads Used in Assembly 45,449 14,377 34,672 nd 4,216,721
# bp Used in Assembly 21,760,812 10,121,408 nd nd 1,449,059,795
% Raw Reads Assembled 59.79% 99.14% nd nd 99.26%
# Contigs or Clusters 4,550 2,575 4,785 72,977 43,321
N50 7 or Mean Contig Length (bp) 471 935 750 2,275 2,133
# Singletons or # Single ESTs 8 7,611 6,032 7,924 nd 120,805
% Singletons (of assembled reads) 16.75% 41.96% 22.85% nd 2.86%
# Total Assembly Products 12,161 8,607 12,709 72,977 142,317
# Unigenes or # Unique BLAST 9 Hits to nr 12,616 8,607 12,709 11,490 19,874
1

Data from [73], [74].

2

Data from [75].

3

Data from [76].

4

Data from [72].

5

Data from this report.

6

L = larval stage. nd = data not reported in the relevant publication [72], [73], [74], [76].

7

“N50” refers to isotig N50 from the G. bimaculatus de novo transcriptome assembly; mean contig length is shown for all other orthopteran transcriptome resources in this table.

8

# singletons are shown for the G. bimaculatus de novo transcriptome assembly; # single ESTs (not incorporated into contigs) are shown for all other orthopteran transcriptome resources in this table.

9

# unique BLAST hits against nr are shown for the G. bimaculatus de novo transcriptome assembly; # unigenes are shown for all other orthopteran transcriptome resources in this table.