Table 2. Assembly statistics and BLAST results against nr for the G. bimaculatus de novo transcriptome assembly.

Parameter1	Value
# bp Raw reads	1,483,726,666
Maximum raw read length	803
Minimum raw read length	13
Median raw read length	364
Maximum assembled read length	771
Minimum assembled read length	20
Median assembled read length	358
# Isogroups2 (“genes”)	16,456
Mean # isotigs per isogroup	1.2
# Isotigs	21,512
Maximum isotig length	10,865
Minimum isotig length	57
Median isotig length	1,054.5
# Isotigs with BLAST hit against nr 3, E-value cutoff e-10 (% of all isotigs)	11,135 (51.8%)
# Isotigs with BLAST hit against nr, E-value cutoff e-5 (% of all isotigs)	11,943 (55.5%)
Mean # contigs per isotig	1.7
# Singletons	120,805
Maximum singleton length	620
Minimum singleton length	50
Median singleton length	250.5
# Singletons with BLAST hit against nr, E-value cutoff e-10 (% of all singletons)	7,914 (6.6%)
# Singletons with BLAST hit against nr, E-value cutoff e-5 (% of all singletons)	10,815 (9.0%)
# Non-redundant assembly products (NRAP)	142,317
# NRAP with BLAST hit against nr, E-value cutoff e-10 (% of all NRAP)	19,049 (13.4%)
# NRAP with BLAST hit against nr, E-value cutoff e-5 (% of all NRAP)	22,758 (16.0%)
Total # BLAST hits4 (nr)	22,758
Average coverage/bp	51.3

¹Values for number of raw reads, number and % of raw reads assembled (passed quality filters described in main text), number of contigs, isotig N50, % of singletons, total number of assembly products, and number of unique BLAST hits against nr, are shown in Table 1.

²Because isogroups are collections of isotigs that are hypothesized to originate from the same gene, they do not comprise a single sequence and so cannot be mapped to nr using BLAST.

³ nr = NCBI non-redundant database.

⁴For BLAST against nr the E-value cutoff was 1e-5. For breakdown of BLAST hits among different classes of assembly sequences, see Table 3.