Table 5. Statistical comparison of isotig and singleton nucleotide sequence lengths according to BLAST annotation and predicted protein-coding status.
| BLAST hit1/predicted protein coding status2 | Significant hit against nr2 | Significant hit against nr and contains predicted coding regions | No significant hit against nr | No significant hit against nr and contains predicted coding regions | All NRAS3 containing predicted protein-coding regions | All NRAS without predicted protein-coding regions | No significant hit against nr and significant hit against Locusta migratoria sequences | No significant hit against nr and significant hit against Laupala kohalensis sequences |
| ISOTIGS 4 | ||||||||
| Significant hit against nr | 0.9998 | *** | *** | *** | *** | *** | *** | |
| Significant hit against nr and contains predicted coding regions | 1 | *** | 1 | 1 | 1 | 1 | ||
| No significant hit against nr | 1 | 1 | *** | 1 | 1 | |||
| No significant hit against nr and contains predicted coding regions | *** | 1 | *** | *** | ||||
| All NRAS containing predicted protein-coding regions | *** | *** | *** | |||||
| All NRAS without predicted protein-coding regions | 1 | 1 | ||||||
| No significant hit against nr and significant hit against Locusta migratoria sequences | 0.2268 | |||||||
| No significant hit against nr and significant hit against Laupala kohalensis sequences | ||||||||
| SINGLETONS | ||||||||
| Significant hit against nr | 0.9798 | *** | *** | *** | *** | *** | *** | |
| Significant hit against nr and contains predicted coding regions | 1 | *** | 1 | 1 | 1 | 1 | ||
| No significant hit against nr | *** | 1 | *** | 0.4208 | 1 | |||
| No significant hit against nr and contains predicted coding regions | *** | *** | * | *** | ||||
| All NRAS containing predicted protein-coding regions | *** | *** | 0.0969 | |||||
| All NRAS without predicted protein-coding regions | 0.1358 | 0.9985 | ||||||
| No significant hit against nr and significant hit against Locusta migratoria sequences | 0.9967 | |||||||
| Significant hit against Laupala kohalensis sequences | ||||||||
Values shown are p≥0.05 value results of a Welch's t-test.
***
= p<0.0001;
*
p<0.05.
1
BLAST E-value cutoff is e-5 for all hits reported in this table.
2
nr = NCBI non-redundant database.
3
NRAS = all non-redundant assembly products (isotigs or singletons) regardless of BLAST results against nr.