Table 6. Statistical comparison of isotig and singleton predicted coding sequence lengths according to BLAST annotation status.

BLAST hit1/predicted protein coding status2	Significant hit against nr2	No significant hit against nr	All NRAS3	No significant hit against nr and significant hit against Locusta migratoria sequences	No significant hit against nr and significant hit against Laupala kohalensis sequences
ISOTIGS 4
Significant hit against nr		***	1	**	***
No significant hit against nr			***	1	1
All NRAS				*	0.0059
No significant hit against nr and significant hit against Locusta migratoria sequences					0.4052
No significant hit against nr and significant hit against Laupala kohalensis sequences
SINGLETONS
Significant hit against nr		***	1	***	***
No significant hit against nr			***	1	1
All NRAS				0.4091	0.9235
No significant hit against nr and significant hit against Locusta migratoria sequences					0.8685
No significant hit against nr and significant hit against Laupala kohalensis sequences

Values shown are p≥0.05 value results of a Welch's t-test.

^*** = p<0.0001;

^** p<0.001;

^* p<0.05.

¹BLAST E-value cutoff is e-5 for all hits reported in this table.

² nr = NCBI non-redundant database.

³NRAS = all non-redundant assembly products regardless of BLAST results against nr.

⁴Numbers of sequences in each category are shown in Figure 9. Mean, median, maximum and minimum values for each category are shown in Tables 3 and 4.