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. 2013 May 6;8(5):e63737. doi: 10.1371/journal.pone.0063737

Figure 1. Detection of full-length or truncated NS80 by Western blotting.

Figure 1

A. Diagram of NS80 N-terminal truncations. The two predicted coil and intercoil regions are indicated. Full-length (742) or truncated fragments (Δ55–Δ614) were fused with FLAG tags and then inserted into the pcDNA3.1 vector. C, cytoplasmic; N, nucleus. B, C, D, E. Western blot analysis of expression products of full-length and truncated NS80 in cells transfected with anti-FLAG antibodies. Full-length or N-terminal truncated NS80 is indicated at the top of the figure. The detections (B, C, and D) were carried out using 12% SDS-PAGE and the detections (E) were carried out using 15% SDS-PAGE.