A. Diagram of NS80 regions required for associations with VP1, VP2, VP3, VP4, and NS38. aa 1–55 of NS80 and aa 20–39 of NS38 were responsible for interactions with each other, and aa 56–85 of NS80 were responsible for interactions with VP3. aa that are required for associations with VP1, VP2, and VP4 belonged to regions 550–742 of NS80. The intercoil region is important for self-aggregation and nuclear localization. B. Diagram of MRV μNS regions required for interactions with λ1, λ2, λ3, μ2, σ2, and σNS. aa 1–12 of μNS were responsible for interactions with σNS. aa 20–25 were responsible for interactions with μ2. aa 65–75 and aa 75–85 were responsible for interactions with λ1 and λ2. aa 173–221 were responsible for interactions with σ2, and aa 471–721 were required for interactions with λ3. The intercoil region of μNS is important in self-aggregation.