Table 1. Summary of N-terminal truncations of NS80.

Truncations	The remaining residues	Protein Size (kDa)	Form inclusions		Coils and intercoil			Interactions with other proteins
			C	N	Coil 1	Inter coil	Coil 2	VP1	VP2	VP3	VP4	VP5	NS31	VP6	NS38	VP7	NS25
NS80	742	80	•	○	√	√	√	+	+	+	+	−	−	+	+	−	−
Δ 55	687	75.6	•	○	√	√	√	+	+	+	+				−
Δ 85	657	72.2	•	○	√	√	√	+	+	−	+				−
Δ 126	616	67.8	•	○	√	√	√	+	+	−	+				−
Δ 216	526	57.8	•	○	√	√	√	+	+	−	+				−
Δ 283	459	50.5	•	○	√	√	√	+	+	−	+				−
Δ 373	369	40.6	•	○	√	√	√	+	+	−	+				−
Δ 417	325	35.6	•	○	√	√	√	+	+	−	+				−
Δ 491	251	27.6	•	•	√	√	√	+	+	−	+				−
Δ 499	243	26.7	•	•	√	√	√	+	+	−	+				−
Δ 504	237	26.2	•	•	√	√	√	+	+	−	+				−
Δ 512	230	25.3	○	•	√	√	√	+	+	−	+				−
Δ 549	193	21.2	○	•	×	√	√	+	+	−	+				−
Δ 614	128	14.1	Diffusely		×	×	√

C: cytoplasm.

N: nucleus.

•: located in the cytoplasm or nucleus.

○: not located in the cytoplasm or nucleus.

√: had coils or intercoil.

×: had no coils or intercoil.

+: interactions with other proteins.

−: no interactions with other proteins.

blank grid: not tested.