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. 2013 May 6;8(5):e62591. doi: 10.1371/journal.pone.0062591

Figure 5. Infected mouse lung extracts (IMLE) HPLC- single ion monitoring (SIM)MS ion chromatograms.

Figure 5

HPLC-SIMMS analysis of crude mouse lung extracts corresponding to mice infected with wild type, or OErsmA. (A) Ion chromatogram showing compound 6 is approximately two to three times as abundant in the OErsmA-IMLE relative to WT-IMLE. (B) Similarly, gliotoxin is about two times as abundant in OErsmA-IMLE than WT-IMLE. Reference chromatograms (bottom panels) show diagnostic ions of cyclo(L-Phe-L-Ser) (6) and gliotoxin (1). Lung extract chromatograms are scaled to the peaks measured in the OErsmA-IMLE sample (bottom panels of standards are not to scale).